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Inflammatory factor expression Fasting venous serum samples were collected on preoperative day 1 and postoperative day (POD) 3 to detect tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, and prostaglandin E 2 (PGE2) expression levels using enzyme immunoassays (R&D Systems, Inc., Minneapolis, Minnesota, USA)

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The intraindividual variability (mean squared difference) of a trainee will be compared with that of the gold standard